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The European University Initiative aims to strengthen innovation across Europe through transnational alliances. Yet limited understanding exists of how universities within alliances can collaboratively develop innovation and entrepreneurship ecosystems. While research on Third Mission activities focuses on university-industry transfer, inter-university collaboration remains underexplored. This exploratory case study examines EELISA, analyzing strategic documents and interviews with entrepreneurship coordinators from ten member universities. Findings reveal that alliances serve as valuable platforms for mutual learning and joint entrepreneurial educational development. However, integrating regional ecosystem partners and achieving genuine complementarity beyond knowledge exchange remain significant challenges.

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